Microbe Isolation/Detection - RAN Biotech - Beverly, MA

AFFINITY MATERIALS FOR ISOLATION OF MICROBES AND A NOVEL TECHNIQUE FOR THEIR POU DETECTION

You have to know – when microbial presence is a risk to safety, to efficiency, to functionality or when it is part of your research – then capturing and isolating them and performing diagnostic tests to measure their presence is essential. But culture-plate methods take days, and many rapid microbe isolation and detection methods require a capital investment and a higher cost per test.

Not with RAN Biotech’s materials and methods. RAN Biotech’s patented approach captures, isolates and detects microbes accurately, affordably – and in hours instead of days. It is based on novel nextgen affinity beads and resins capable of capturing and isolating microbes including viruses and if needed, aggregating them in the form of a pellet that separates from the rest of the sample via various methods, e.g. gravity, filtration or magnetism. Our technology works in samples as small as 0.03 nano-liter micro-reactors and droplets and up to liters.

Try It

A better way to isolate and detect microbes

You’ve got a lot of options for so-called “rapid microbiological methods” – but our materials and methods are unique to RAN Biotech. Our clients are finding it’s a powerful complement or even a replacement for their current materials that manipulate microbes and methods that detect them.

Fast results – minutes or hours

Low cost-per-test

Visual results

Numerical results

Highly sensitive

Field-ready in case of POU microbe detection

Easy to learn

Non-destructive

Tested samples can be used in further downstream analyses

Case Study 1: Isolating HIV-1

Two types of RAN Biotech resins (RMD1 and RMD2) are added to media samples infected with HIV-1. The suspensions are allowed to mix for 20 minutes before separating the captured viruses by gravity or a quick centrifugation. Similar results were obtained when testing infected plasma and whole human blood.

Case Study 2: Microbial Analysis of Environmental Samples

Metagenomic studies on microbes isolated from small volume environmental samples show sequence signatures from many different microbes including several human viruses. Water samples isolated from the Mississippi River were mixed with RAN Biotech’s resins. The samples were then stabilized by adding an equal volume of RNAlater. High molecular weight gDNA was extracted and Nextera sequencing libraries were created. Samples were multiplexed and sequenced using the Illumina MiSeq platform. Sequencing reads were analyzed by the DRAGEN and Kraken pipelines.

Case Study 3: RAN Biotech’s POU Microbial Detection

Visual, numerical results – in hours, not days

RAN Biotechnologies microbe capturing and isolating materials are being applied to a novel POU microbe detection technique. It is an affordable, timely alternative that can replace your bioscan, ATP testing or culture-plate methods. Unlike most so-called rapid microbiological methods, RAN Biotechnologies technique is portable enough to use in the field. And it’s simple enough for almost anyone to use.

Here’s how it works.

1. Sample and Mix

Add a drop of RMD affinity nanomaterials to your test sample, then mix. If microbes and viruses exist in your sample, RAN Biotech’s patented materials isolate them without destroying them, forming an RMD Pellet that sinks to the bottom of your sample, or that could be separated via filtration or magnetism.

2. View and Quantify

The RMD Pellet is visible – just use your smartphone and RAN Biotech’s handheld detector. The size of the pellet and the speed of its formation indicate the starting titer of microbes in your sample.

You can digitize, evaluate and save RMD findings with RAN Biotech’s RMD software. Microbial capture and isolation works on any sample size – but the larger your sample, the more sensitive your test.

And because microbial measurement is non-destructive, you can have samples with positive RMD results further tested for identification of specific microbial strains.

Microbe Isolation and Detection