OrchestRANum™ System - RAN Biotech - Beverly, MA

Single Cell RNA-Seq Enabled by the OrchestRANum™ System

Precise, Efficient, and Cost-Effective Generation of BARcodes in Droplets (BARDs™)

Since 2012, RAN Biotechnologies has supplied the materials used in many of the foundational studies of droplet-based single-cell research. OrchestRANum™ builds on this expertise by bringing these proven components together in a streamlined, ready-to-run system, no microfluidics experience required.

When paired with high-diversity barcoding, the system enables single-cell mRNA sequencing with exceptional resolution and flexibility, empowering researchers to uncover gene expression dynamics with precision and at a fraction of traditional costs.

A Simplified Path to Single-Cell Resolution

The OrchestRANum™ platform was designed for simplicity, speed, and performance, allowing researchers to generate high-quality single-cell libraries on a small footprint and budget.

Each system includes the Partitioning Station and pre-packaged reagent kits containing all necessary materials to generate BARDs™ and encapsulate a defined number of cells, from preparation to completion in just minutes to hours. Researchers simply provide their cells of choice and preferred reverse transcriptase.

Compact. Intuitive. Reliable.

The USB-powered Partitioning Station is PC-controlled and features a compact 4-channel fluidic system capable of managing up to four reagents simultaneously, and efficiently co-encapsulating barcodes with single cells.

Built for both academic and industrial labs, the system allows teams to scale single-cell studies without the expense or rigidity of closed, proprietary platforms.

Key Benefits

Reliable Results: Achieve 60-100% encapsulation efficiency (cell-to-bead ratio) depending on cell size.
Flexible Integration: Seamlessly fits into user-defined workflows and assay types.
Time-Efficient: Typical setup and run time is about one hour (assuming cells are in suspension).
Cost-Effective: Achieves high cell recovery efficiency while reducing reagent consumption
Simplified Analysis: High single-cell/single-bead capture rates minimize data complexity.

Achieving Single Cell Resolution with BARD™ Technology

The Single-Cell and Barcode-Bead Co-Encapsulation (CoE) System is optimized for use with pre-packaged kits that accommodate different experimental scales based on the starting number of cells.

Each kit includes:

Barcode beads
Partitioning and Recovery reagents
Partitioning Cassettes

The kits support generation of specific number of BARDs™ for encapsulating single cells.

The 10MINKIT is designed for processing a total of 40,000 cells.
The 20MINKIT supports a total of 80,000 cells.

Each kit includes reagents in slightly excess volumes for user optimization and a detailed, step-by-step User Guide. Researchers can readily aliquot reagents for smaller scale experiments or pilot runs, allowing maximum flexibility across different cell input numbers and project sizes.

Single-Cell and Barcode-Bead Co-Encapsulation Process

Typical Result Profile

Specifications

Barcode Beads (RANscBead-X2):

Gel beads loaded with ~1.7 million unique barcodes, each functionalized with a poly(T) sequence for direct mRNA capture from lysed single cells.

Structure of Barcode:

Bead Storage: Maintain refrigerated at 2-4°C.

User Supplied Materials:

Laptop, Collection tubes,100% Ethanol, Cell culture, Tabletop Microcentrifuge, Hemocytometer

Choosing the Right Single-Cell Platform for Your Lab

Ordering Information

Product ID	Description
OrchestRANum™ System	– 4-channel micro-pump system with Windows-compatible control software – Validated, ready-to-run protocol for consistent droplet generation
10MINKIT	Optimized for processing a total of 40k cells. Includes: – 500k barcode beads – 0.5mL Partitioning Reagent – 0.5mL Recovery Reagent – Partitioning Cassettes (8 devices total)
20MINKIT	Optimized for processing a total of 80k cells. Includes: – 1M barcode beads – 1mL Partitioning Reagent – 1mL Recovery Reagent – Partitioning Cassettes (16 devices total)