Temporal recording of mammalian development and precancer
Published: October, 2024
The researchers developed NSC-seq, a single-cell CRISPR platform that concurrently captures mRNA and guide RNAs, allowing cellular state, lineage and temporal information to be studied together. Published in Nature, their work provides new insights into mammalian development and the origins of colorectal precancer.
Custom multiomic hydrogel beads produced by RAN Biotechnologies were used in this work.
Applications of OrchestRANum™ System: Single-Cell BARD™ Production
Published: October, 2025
High-quality single-cell RNA sequencing begins with reliable partitioning and barcoding. This application note highlights how the OrchestRANum™ System enables the production of Barcode Droplets (BARD™) by pairing individual cells with unique barcode beads in stable microfluidic droplets for downstream transcriptomic analysis.
The note outlines a flexible droplet-based workflow designed to support single-cell applications while emphasizing key experimental considerations such as cell preparation, dilution, and assay design. Built on proven microfluidic principles and customizable components, OrchestRANum™ provides researchers with an accessible platform for generating high-quality single-cell partitions.
Contact us to request the complete application note and discover how OrchestRANum™ can accelerate your single-cell research.
Applications of OrchestRANum™ System: Partitioning of Matrigel® 3D Scaffolds
Published: September, 2025
Three-dimensional (3D) cell culture models are transforming biological research by providing environments that more closely resemble native tissues than conventional 2D systems. In this application note, we demonstrate how the OrchestRANum™ System can be rapidly adapted to generate millions of microscale Matrigel® partitions, creating uniform 3D scaffolds suitable for cell culture, viability studies, and screening applications.
By combining off-the-shelf hardware, optimized consumables, and flexible protocols, OrchestRANum™ streamlines the implementation of droplet microfluidics and reduces the need for extensive system development. The result is a practical platform for researchers seeking scalable, customizable, and high-throughput 3D partitioning workflows.
Interested in learning more? Contact us to request the complete application note and explore how OrchestRANum™ can support your 3D culture applications.
CustomSeqReady™ Beads. Flexible Barcoding for Your Research
Published: April, 2025
Barcoded bead synthesis has traditionally come with high upfront costs and large minimum order requirements—making early-stage testing costly and inflexible.
At RAN Biotech, we have changed that.
CustomSeqReady™ Beads come pre-functionalized with barcodes and a ready-to-use adaptor, allowing researchers to easily add their capturing sequences without needing to invest in full-scale custom syntheses.
This modular approach gives users:
– Lower entry costs
– Full customization freedom
– Scalable options as your project grows
Whether you’re prototyping or scaling, CustomSeqReady™ beads make precision barcoding more accessible than ever.
Contact us for more information or to request a sample.
PFAS-Free Consumables for Droplet Microfluidics
Published: October, 2024
RAN Biotech is excited to launch a new line of PFAS-free consumables—designed specifically for water-in-oil droplet microfluidic applications. This innovative lineup includes a continuous oil phase, surfactant, emulsion-breaking oil, and coated PDMS microfluidic devices.
Engineered for the rigorous demands of high-throughput droplet-based biological studies, our PFAS-free consumables deliver precision, reliability, and sustainability, providing researchers with flexible solutions for all their droplet microfluidic applications.
Explore the possibilities with RAN Biotech’s newest innovations!
RANscBEAD-X1: Affordable Single Cell Barcode Bead
Published: July, 2024
The RANscBead-X1 Barcode Gel Bead is a game-changing solution offering unparalleled affordability and reliability, making high-quality single cell analysis accessible like never before. A perfect match for the pioneering single cell researcher eager to push the boundaries of science without breaking the bank!
GEL BEADS: The Tunability of Polyacrylamide Bead Diameter
Published: May, 2024
By simply varying the type of buffer used, RAN Biotech’s team has explored methods to finely tune the diameter of polyacrylamide gel beads, enhancing their loading into picoliter-sized droplets in confined microfluidic devices.
Dissociation and inDrops microfluidic encapsulation of human gut tissues for single-cell atlasing studies
Authors: Alan J. Simmons, Ken S. Lau
STAR Protocols (2022), Vol 3 (3), pp 101570
Published: September 16, 2022
Massively multiplexed nucleic acid detection using Cas13
Authors: Cheri M. Ackerman, Cameron Myhrvold, Sri Gowtham Thakku, Catherine A. Freije, Hayden C. Metsky, David K. Yang, Simon H. Ye, Chloe K. Boehm, Tinna-Sólveig F. Kosoko-Thoroddsen, Jared Kehe, Tien G. Nguyen, Amber Carter, Anthony Kulesa, John R. Barnes, Vivien G. Dugan, Deborah T. Hung, Paul C. Blainey & Pardis C. Sabeti
Nature (2020), Vol 582, pp 277-282. ASAP, DOI: https://doi.org/10.1038/s41586-020-2279-8
Published: April 29, 2020
Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells
Authors: Allon M. Klein, Linas Mazutis, Ilke Akartuna, Naren Tallapragada, Adrian Veres, Victor Li, Leonid Peshkin, David A. Weitz, Marc W. Kirschner
Cell (2015), Vol 161 (5), pp 1187-1201
Published: May 21, 2015
In vivo quantification of spatially-varying mechanical properties in developing tissues
Authors: Friedhelm Serwane, Alessandro Mongera, Payam Rowghanian, David A. Kealhofer, Adam A. Lucio, Zachary M. Hockenbery, Otger Campàs
Nature Methods (2017), Vol 14 (2), pp 181-186
Published: July 24, 2017
A Barcode-Free Combinatorial Screening Platform for Matrix Metalloproteinase Screening
Authors: Tushar D. Rane, Helena C. Zec, Tza-Huei Wang
Analytical Chemistry (2014), ASAP, DOI: 10.1021/ac504330x
Published: December 28, 2014
Rapid Enumeration of Phage in Monodisperse Emulsions
Authors: Katrina F. Tjhung, Sean Burnham, Hany Anany, Mansel W. Griffiths, and Ratmir Derda
Analytical Chemistry (2014), DOI: 10.1021/ac500244g
Published: May 20, 2014
Testing Turing’s theory of morphogenesis in chemical cells
Authors: Nathan Tompkins, Ning Li, Camille Girabawe, Michael Heymann, G. Bard Ermentrout, Irving R. Epstein, Seth Fraden
Proceedings of the National Academy of Sciences of the USA (2014), DOI: 10.1073/pnas.1322005111
Published: March 10, 2014
Removing human immunodeficiency virus (HIV) from human blood using immobilized heparin
Authors: Roger A. Nassar, Edward P. Browne, Jianzhu Chen, Alexander M. Klibanov
Biotechnology Letters (2012), Vol 34 (5), pp 853-856
Published: December 30, 2011
